MadSci Network: Botany

Re: What chemicals are needed in the vitrification process of plant tissue?

Date: Tue Sep 26 09:49:44 2006
Posted By: David Hershey, Faculty, Botany, NA
Area of science: Botany
ID: 1159224134.Bt

The article by Panis and  Lambardi (2005) lists the standard solution, Plant
Vitrification Solution #2, as 30% glycerol, 15% ethylene glycol and 15%
dimethylsulfoxide (DMSO) (all on a volume basis) in a tissue culture medium
containing 0.4 M sucrose.

A high school science teacher could probably obtain those chemicals. The
vitrification technique involves use of tissue-cultured or in vitro plantlets.
Liquid nitrogen is used for the freezing. The article discusses various other
plant cryopreservation techniques.  

For more information, do a search on vitrification plant

You may be able to find further assistance from a university or government
agency conducting cryopreservation research, such as the USDA Plant Germplasm
Preservation Research Unit in Fort Collins, CO. 

Vitrification is a confusing term in plant tissue culture or plant
micropropagation because it is defined in two different ways. Originally, it
referred to abnormal plant organs or tissues but the preferred term for that is
now hyperhydricity. The preferred definition for vitrification is the change
from liquid to solid state during cryopreservation (Debergh et al. 1992).


Panis, B. and Lambardi, M. 2005. Status of cryopreservation technologies in
plants (crop and forest trees). pp. 61-78, Part II IN: The Role of Biotechnology
in Exploring and Protecting Agricultural Genetic Resources. Rome: FAO.

Debergh, P., J. Aitken-Christie, D. Cohen, B. Grout, S. von Arnold, R. Zimmerman
and M. Ziv.1992. Reconsideration of the term ‘vitrification’ as used in
micropropagation. Plant Cell, Tissue and Organ Culture 30: 135-140.

USDA Plant Germplasm Preservation Research Unit

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