Re: How do we standardized drug concentration for cell lines?

Unfortunately, if there is no previous data on the concentration limit, 
you have to perform serial dilutions and check cell viability. I assume 
you are using Geldanamycin, or a derivative (17-AAG17-DMAG)
Geldanamycin causes a dose-dependent G2 arrest and reversible inhibition 
of entry into the S phase in studied cancer cells at 0.1 rwM, which is a 
pretty high concentration.  Since the arrest is reversable, I'd suggest 
initial 10 fold dilutions (starting with 10mM and getting weaker), and 
see when the cells don't arrest.  I know this is a pretty high amount to 
start with, but HSP90 is relatively abundent in the cell. Sorry I don't 
have more information, but it's pretty much trial and error if it hasn't 
been done before.  I also found a link that might help. http://dtp.nci.nih.gov/branches/btb/ivclsp.html