MadSci Network: Botany

Re: What colors could I see in the chromatogram besides green turquoise and ora

Date: Wed Oct 11 15:48:37 2006
Posted By: David Hershey, Faculty, Botany, NA
Area of science: Botany
ID: 1160522450.Bt

Chlorophyll c and fucoxanthin are not found in spinach. Fucoxanthin is a brown
pigment found in brown algae and diatoms. Chlorophyll c is only found in the
Chromista and dinoflagellates. 

Lutein is a carotenoid, specifically a xanthophyll. It is present in spinach and
is yellow to orange-red. The USDA National Nutrient Database for Standard
Reference says there are 12,198 mcg of lutein + zeaxanthin per 100 grams of raw

Sometimes there are additional bands in paper chromatography, such as an
olive-green band of pheophytin (see last reference).

This is a standard lab exercise so try a search for: paper
chromatography spinach.


Photosynthetic Pigments


USDA National Nutrient Database for Standard Reference

Re: How do you identify pigments as they show up on paper chromatography?

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