MadSci Network: Molecular Biology
Query:

Subject: recombinant dna- why must the cuts be so close to the dna gene?

Date: Thu Oct 26 20:54:05 2006
Posted by blenda
Grade level: undergrad School: ga highlands
City: rome State/Province: ga Country: usa
Area of science: Molecular Biology
ID: 1161921245.Mb
Message:

there is no explanation of why there must be cuts close to the gene as possible-
without cutting the gene itself-just that it must be. I'm assuming it will not
give the end result needed for proper cloning. So-will it make a defective dna
clone, or just too many or too little? This is concerning enzyme restriction a
plasmid and a dna gene of insulin.


Re: recombinant dna- why must the cuts be so close to the dna gene?

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