MadSci Network: Molecular Biology |
Hi, Im a lab technician, I am doing PCRs... I am often confronted to PCR inhibitors. I could figure out (litterature) what they are : high molecular weight sugars that are co-purified with the DNA/RNA. They come from plants and are found in fecal animal samples. They interfere with the PCR process (I do not know if they stick to the DNA or inhibit the enzyme itself...). I cannot find a protocol that can destruct these sugars. What kind of chemical reaction would destroy them without harming the DNA ? Thank you in advance
Re: PCR inhibitors - how to get rid of them ?
Try the links in the MadSci Library for more information on Molecular Biology.