MadSci Network: Molecular Biology
Query:

Subject: PCR inhibitors - how to get rid of them ?

Date: Tue Oct 31 09:33:20 2006
Posted by Seb
Grade level: undergrad School: LNS-Immunology
City: Arlon State/Province: No state entered. Country: Belgium
Area of science: Molecular Biology
ID: 1162312400.Mb
Message:

Hi, 

Im a lab technician, I am doing PCRs... I am often confronted to PCR inhibitors.
I could figure out (litterature) what they are : high molecular weight sugars
that are co-purified with the DNA/RNA. They come from plants and are found in
fecal animal samples. They interfere with the PCR process (I do not know if they
stick to the DNA or inhibit the enzyme itself...). I cannot find a protocol that
can destruct these sugars. What kind of chemical reaction would destroy them
without harming the DNA ?

Thank you in advance


Re: PCR inhibitors - how to get rid of them ?

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