|MadSci Network: Biochemistry|
This year for my science fair project i wanted to do it on the digestions of protiens in the stomach. The description of the project was to use Ninhydrin Isopranol in a test tube with a buffer solution and the protien Albumin While using different PH levels( from 4-10). However, the buffer solution was not specified as to what the chemical components are. I was wondering if you knew what buffer solution i could use to get a color change an an absorbance level when using a spectrophotometer set to 580 nm after we have used a centrifuge. For more detailed understanding..this is the abstract I took my science fair project idea from : The purpose of this porject was to observe how the digestion of protien varies at different PH levels. A portien, albumin, was placed into four test tubes for each of the seven PH levels being used. Then, the buffer solution for each PH level was placed into their respective tubes. After a period of ten minutes, Ninhydrin Isopranol was placed in one of the tubes for each PH leve. Thi step was repeated every ten minutes. Then after being centrifuged, the solutions were observed for color change and the absorbance was recorded using a spectrophotometer at 580 nm. if there are more details needed for you to answer my question please email me firstname.lastname@example.org thank you very much ps. I need an answer ass soon as possibly thx
Re: What buffer solution is needed to decompose the protien albumin?
Try the links in the MadSci Library for more information on Biochemistry.