MadSci Network: Cell Biology

Re: Are the effects of triton x-100 temporary?

Date: Wed Jan 10 06:55:04 2007
Posted By: Sebastien De Landtsheer, Undergraduate, Immunology, Laboratoire National de Santé
Area of science: Cell Biology
ID: 1167433465.Cb


To my knowledge, Triton x-100 is a quite aggressive compound. It makes big
holes in the membranes and degradates some proteins. The litterature is
well documented, see Deamer and Crofts Journal of Cell Biology, Vol 33,
395-410 for example...
But there is no way to reverse the mechanism. I'm afraid your paramecium
will be somehow killed by this process, unless you use very small quantities.
A compond that closes membranes ? I know PEG (polyethyleneglycol) that is
used to fuse cells together, and some viruses have this action too...

Hope this helps...

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