MadSci Network: Molecular Biology
Query:

Subject: what are the various factors that can affect the activity of restriction en

Date: Sat Dec 30 04:49:54 2006
Posted by jane
Grade level: teacher/prof School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Molecular Biology
ID: 1167479394.Mb
Message:

what are the various factors that can affect the activity of restriction 
enzymes?if,a sample of DNA has protein contamination then will this affect 
restriction digestion?can addition of proteinaseK in a larger amount than 
required will hinder the R.E activity in any case?


Re: what are the various factors that can affect the activity of restriction en

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