|MadSci Network: Biochemistry|
Hello, I am a junior who is currently conducting a science project on caspases. I would like to know how I can determine the specific amino acid sequence in which caspases show affinity for (or cleave after). Which programs can I use to determine this? I did a secondary experiment to measure YCA1 (a putative yeast caspase) activity in S. cerevisiae. The protein has already been discovered as a regulator of apoptosis in yeast and the Saccharomyces genome has already been sequenced. I have been told that tBLASTn can determine the sequence of YCA1 but are there any other programs available and how can one carry out this procedure? Thankyou
Re: How can I determine the amino acid sequence for caspase proteolysis?
Try the links in the MadSci Library for more information on Biochemistry.