|MadSci Network: Physics|
We have some problems with ELISA reader (spectrophotometer): we are not sure if filters of 405, 450 and 492 are on right positions. We measured one sample (of yellow color) at supposed 405, 450 and 492 nm and found absorbances: 2,400 ; 1,417 ; 0,829. Is it possible to connect different absorbances of the same sample with different waveleghts? For example absorbance 2,400 was read at wavelength 450 nm? With best wishes! Lilijana Merljak
Re: Connection between wavelength 405, 450, 492nm and absorbances 2,4;1,417;0,8
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