MadSci Network: Biochemistry

Subject: rate of reaction equation for hydrogen peroxide and catalase.

Date: Sun Mar 25 03:49:51 2007
Posted by Amirah
Grade level: 10-12 School: Greenhead High School
City: No city entered. State/Province: No state entered. Country: England
Area of science: Biochemistry
ID: 1174819791.Bc

for my assignment i need to look at how the rate of reaction is affected when 
the concentration of the substrate (hydrogen peroxide) is changed in the 
reaction between Hydrogen Peroxide and Enzyme catalase.
basically in my experiment i looked at how much oxygen was produced by looking 
at the reading on the burette. i composed a table etc. however how do you show 
the rate of reaction?
i have checked in madsci archive and found the equation:
 reaction rate = 
(Vmax) x [Concentration of hydrogen peroxide] x [Enzyme concentration] 
               Km + [Concentration of hydrogen peroxide] 

but i am finding it difficult to understand what i will put in the (vmax) 
value, it was described as the max rate of reaction but how do i find that out 
when i dont know any rate of reaction? i'm confused.

Re: rate of reaction equation for hydrogen peroxide and catalase.

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