|MadSci Network: Molecular Biology|
Hello Ann, I doubt that the heating and rendering of the animal foods would degrade the mtDNA to the point that it would not be recognized. Firstly, the primers that are used recognize small snipets of DNA that would survive the processing. Secondly, the pcr process that was utilized did recognize bovine and swine DNA in the foods. It's doubtful that the primer cocktail that they used would selectively inhibit one species of DNA from amplifying. Also, notice that pentobarbital was found in 2 of 4 samples of food with no evidence of mammalian DNA. This indicates to me that the source of the pentobarbital is from something else than animal remains (contamination from cleaners used on the equipment?) Best Regards, Dale L. Laux Forensic Scientist
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