MadSci Network: Development

Re: what's markers to identify for Mesenchymal stem cell from human cord blood?

Date: Mon Jun 25 15:20:33 2007
Posted By: William Gunn, Grad student, The Center for Gene Therapy, Tulane University
Area of science: Development
ID: 1179974139.Dv

Huynh -

I work in a lab that studies multipotent cells from bone marrow stroma. We have some panels(sets of antibodies) that we use for various diagnostic purposes. These usually include CD36, 34, 19, 11b, 14, 49b, 105, 117, and sometimes HLA-DR.

These markers are used for analysis of cells from bone marrow. I've never done cord blood, but it's important to note that the markers are for analysis of MSCs, but not for isolation. The current best practice for isolation is still culture of mononuclear adherent cells on tissue culture plastic.

For bone marrow, we've not found a set of cell-surface antigens that allows isolation of a population identical to that produced by purification via culture plate adherence.

It's also important to note that the cells many people call "mesenchymal stem cells" aren't actually mesenchymal, and may not be "stem" either. Better to call them multipotent progenitor cells than mesenchymal. Clarification of the nomenclature is discussed in Cytotherapy, Volume 7, Issue 5 November 2005 , pages 393 - 395, however, I believe the "mesenchymal" part may be dropped as well.

Best luck with your research.

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