Subject: What is the necessary g force for isolating mitochondria in a centrifuge?

I do experiments in my free time, which is why I'm asking this. Is it possible
to run a centrifuge (an isopycnic separation) at a lower RCF (g force) for
longer and get the same level of separation as if you ran it at a higher RCF for
less time. Most of the literature I have read regarding mitochondria separations
suggest 50,000xg or thereabouts for two hours, but I only have access to a
centrifuge with an RCF of up to 20,000xg. Therefore, could I run the separation
in my centrifuge for longer and still separate the mitochondria? -Thank-you.

Re: What is the necessary g force for isolating mitochondria in a centrifuge?