How do you determine how much of a gene to sequence when genotyping?

Subject: How do you determine how much of a gene to sequence when genotyping?

Date: Mon Jul 9 10:00:47 2007
Posted by Meghan
Grade level: grad (science) School: UC Berkeley
City: Berkeley State/Province: CA Country: USA
Area of science: Genetics
ID: 1184000447.Ge

Message:


I'm performing a genetics study that relates certain genes responsible for sleep/circadian function with 
certain mental illnesses. I'm not sure how to determine how much of these genes (portions vs entire gene) 
to sequence. Also not sure how to extract DNA and perform PCR on saliva samples - any suggestions 
would be great! Also, any ideas on potential costs would be great too.

Re: How do you determine how much of a gene to sequence when genotyping?

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