MadSci Network: Genetics |
Dear Traci, From the question, it seems that you have not understood transgenic mice at all. Basicly, if the gene is introduced into the germ line, the whole mouse glows (i.e. all cells), depending on the promoter. The question is thus : is the promoter active in all cell lines ? Something like SV40 or CMV would be active in almost all cell types. Now, provided it is stably introduced, there should be no loss as individual cells reproduce, and the promoter should stay active over time. Theoretically, once a mouse glows, it should glow all its life, and it should have FLK (normally FLK = funny looking kids, but this time Fluorescent Kids) If you have not made a stable line, but injected GFP after provoked a muscle injury, then I would be surprised that ALL the mouse glows, and even if yes, you might probably have loss of fluorescence over time... Hope that helps Regards,
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