MadSci Network: Molecular Biology

Re: why does pcr use only 30 cycles and not more

Date: Fri Feb 15 09:48:47 2008
Posted By: Sanjida Rangwala, Post-doc, University of Pennsylvania
Area of science: Molecular Biology
ID: 1202821550.Mb

Hello there,

There is no reason why PCR should only be run for 30 cycles. I have done PCR for
as little as 25 cycles and as high as 45 cycles. 30 cycles is usually enough to
amplify enough product to be visualized with ethidium bromide on an agarose gel.
Hope that helps!

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