MadSci Network: Biochemistry |
Hi, I'm using the Lowry Method to look at protein content, which requires a standard curve to find out my sample's protein content. So for the standard curve, I wanted to use the protein concentration as the x-axis and absorbance for the y-axis, since the absorbance is depended on the protein concentration. But I was taught to use absorbance as the x-axis instead, because I will later use the absorbance to calculate the protein content. Which axis should I actually use for protein concentration, and does it matter? Thanks!
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