MadSci Network: Biochemistry
Query:

Subject: Axis for standard curves

Date: Tue Mar 4 00:35:44 2008
Posted by Alle
Grade level: undergrad School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Biochemistry
ID: 1204616144.Bc
Message:

Hi, I'm using the Lowry Method to look at protein content, which requires a 
standard curve to find out my sample's protein content. So for the standard 
curve, I wanted to use the protein concentration as the x-axis and absorbance 
for the y-axis, since the absorbance is depended on the protein concentration. 
But I was taught to use absorbance as the x-axis instead, because I will later 
use the absorbance to calculate the protein content.
Which axis should I actually use for protein concentration, and does it 
matter? Thanks!



Re: Axis for standard curves

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