|MadSci Network: Molecular Biology|
Hello, I've been reading much on the two general protein design techniques: directed enzyme evolution and the rational evolutionary design route. However, I'm having a tough time understanding the former concept. My question is, in a directed evolution experiment, is the entire protein of interest mutated randomly or must the scientist identify a specific sequence of interest (or region in the protein) that they wish to modify/mutate? If the latter is true, how does one come about identifying the specific sequence (not a priori) in a protein with literally hundreds to thousands of bps? Your time and help is deeply appreciated. -Amy
Re: In directed evolution, how are sequences of interest identified?
Try the links in the MadSci Library for more information on Molecular Biology.