why does PCR amplify DNA upto10kb(i've read it in many texts)

Subject: why does PCR amplify DNA upto10kb(i've read it in many texts)

Date: Fri Apr 11 12:58:58 2008
Posted by No name entered.
Grade level: undergrad School: No school entered.
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Area of science: Molecular Biology
ID: 1207943938.Mb

Message:


hi.is it some sort of prodedural constraints,what are the restrictions?and do 
we need to cut DNA with RE's prior to PCR,always?can we utilize the whole 
intact genome in PCR?n i want u to confirm,plz,that its only possible to 
obtain an intact genome from bacteria/viruses since it has single 
chromosome/single DNA molecule and not through those organisms having more 
than one chromosome?thanx

Re: why does PCR amplify DNA upto10kb(i've read it in many texts)

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