How can I digest my target protein in-gel using Endoproteinase Glu-C enzyme

Subject: How can I digest my target protein in-gel using Endoproteinase Glu-C enzyme

Date: Wed May 21 21:18:02 2008
Posted by Anas Abdel Rahman
Grade level: grad (science) School: Memorial University of Newfoundland
City: ST. JOHNS State/Province: NL Country: Canada
Area of science: Biochemistry
ID: 1211429882.Bc

Message:


I am working in de novo sequencing to Tropomyosin and I need to confirm my 
trypsin result using another proteolytic enzyme, and I chose protease V8.
my question is can I digest my sample using protease V8 (In-gel protocol) and 
what is this protocol.

Thanks, Anas

Re: How can I digest my target protein in-gel using Endoproteinase Glu-C enzyme

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