How accurate is a catalase assay done at non-saturating substrate conc?

Subject: How accurate is a catalase assay done at non-saturating substrate conc?

Date: Mon Jul 28 14:54:10 2008
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Area of science: Biochemistry
ID: 1217282050.Bc

Message:


Source of catalase-cell lysate of Streptomyces coelicolor.
Conc of H2O2-30mM.
Assay system used:50microlitre lysate+1ml of 30mM H2O2
Measured decrease in O.D at 240nm.
Observed a decrease in catalase activity of a mutant lysate as compaed to WT.
I didn't do a saturation curve to begin with.
So how significant n reliable is the trend obsrvd since it ws dne at non-
saturatin H2O2 concentration?

Re: How accurate is a catalase assay done at non-saturating substrate conc?

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