Subject: Why can't we use DNA polymerase III for PCR?
Date: Wed Jul 23 09:26:57 2008
Posted by Anisha
Grade level: undergrad
School: No school entered.
City: Mumbai State/Province: No state entered.
Area of science: Molecular Biology
I am familar with the fact that the klenow fragment of DNA pol I from Thermus
aquaticus is used during pcr. The klenow fragement is obtained by removing the
5'-3' exonuclease activity of pol I.
Why not use pol III instead? It does not have 5'-3' exonuclease activity
naturally, so klenow fragment production would be unnecessary. Further it has
Re: Why can't we use DNA polymerase III for PCR?
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