MadSci Network: Molecular Biology

Subject: Why can't we use DNA polymerase III for PCR?

Date: Wed Jul 23 09:26:57 2008
Posted by Anisha
Grade level: undergrad School: No school entered.
City: Mumbai State/Province: No state entered. Country: India
Area of science: Molecular Biology
ID: 1216830417.Mb

I am familar with the fact that the klenow fragment of DNA pol I from Thermus
aquaticus is used during pcr. The klenow fragement is obtained by removing the
5'-3' exonuclease activity of pol I.

Why not use pol III instead? It does not have 5'-3' exonuclease activity
naturally, so klenow fragment production would be unnecessary. Further it has
higher processivity. 

Re: Why can't we use DNA polymerase III for PCR?

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