|MadSci Network: Molecular Biology|
In bacterial transformation: I have the general information that Ca2+ neutralizes the DNA and membrane and therefore enables the DNA to enter the cells. I also know that heat shock increases the permeability of the cell membrane - I guess this is related to heat shock proteins and the heat shock response (? - when I search for heat shock I find this most of the time, but never in relation to transformation). But, how does this increase the permeability, and how are HSP and HSR related? And is the process how the DNA enters the cell known - if, how does it work? Somehow I can't find one source which describes how those processes work together in detail (I only find very general information or very scientific, detailed information about single processes, and I don't have enough knowledge nor comprehension to put them together...). I am writing an essay about bacterial transformation. It would be quite important to know this process in a more detailed way, how they are connected. (I would also be very happy about sources.) Thank you very much!
Re: How do CaCl2 and heat shock work together to make cells competent?
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