How does heat shock work (in bacterial transformation)?

Subject: How does heat shock work (in bacterial transformation)?

Date: Tue Aug 19 12:20:19 2008
Posted by No name entered.
Grade level: 10-12 School: No school entered.
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Area of science: Cell Biology
ID: 1219173619.Cb

Message:


I've already searched the archives of madsci, but didn't find the answer to my
question - many of the questions are related to transformation, but the answers
never go into detail with heat shock. I would like to know how specifically heat
shock works in bacterial transformation (of e.coli). How does it increase
transformation efficiency, how does it make cells competent and helps plasmids
to enter the bacteria? And why is the temperature of heat shock most of the time
42°C? So generally I would like to know: how does heat shock work? Thanks!

Re: How does heat shock work (in bacterial transformation)?

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