Re: Why is E. coli's genomic DNA not used for cloning of insulin cDNA?

It is convenient to use plasmids for cloning purposes because of their 
small sizes and thus ease of transformation as compared to genomic DNA. 

However, it is to be noted that many large vectors are also frequently 
used for cloning libraries, for instance, BAC - bacterial artificial 
chromosomes.

Kind Regards,
Tarun Gupta
-- 
MSc Human Genomics 
C/o Prof.Tapas Mukhopadhyay
National Centre for Human Genome Studies and Research   
Panjab University 
Chandigarh-India 

The important thing is not to stop questioning. Curiosity has its own 
reason for existing ~Albert Einstein 

************************************************* 
~About Me: http://hotbacteria.wordpress.com
~Exciting Science forum: http://groups.google.com/group/exciting-science 
~Scientific Information Sharing Resource: http://sisr.blogspot.com 
~My Department: http://nchgsr.puchd.ac.in

*************************************************