| MadSci Network: Microbiology |
Hi Joe!!! Sorry for late reply....I was busy moving to Taiwan from Japan!! OK..let me tell you one thing....When I was in my grad school for my Microbiology Major, We never had incubator for bacterial experiments.... We used to plate the bacteria, wrap them in paper and incubate them in dark in our lockers (we were provided separate lockers for this purpose) What I want to say is, generally all bacteria can grow at room temperature between 25 degree Celsius to 37 degree Celsius..... We even used to grow Escherichia coli (bacteria in our intestine.....used to grow at 37 degree celsius-our body temperature)... SO I think...there is no need to have any sophisticated equipment to carry out simple and basic experiments with bacteria Unless, you stay in a place where room temperature is below 20 degree Celsius.... I also have experience in growing marine bacteria (that grow between 25-29 degree celsius) in lockers in my lab when I was in Japan....where the room temperature was just around 24-26 degree Celsius.... If I had to grow them during winter, I just used to have the heater of the room on and set to around 25 degrees... SO, your method will work...without making any humid atmosphere.....I think there is no need for that.....just keep the petri plates with bacteria in the chamber.....that will just work fine Best of luck in your experiment..... Shashank
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