Subject: How to make 10-120ppm of Chlorpyrifos (Technical grade insecticide) to chec

Background:
I am running an experiment on HPLC to determine that cyanobacteria 
resist/degrade organophosphorous pesticide Chlorpyrifos. I have technical 
grade chlorpyrifos. The problem is that chlorpyrifos degrades to TCP when it 
comes in contact with water as it is sparingly soluble. If i dissolve it in 
methanol/Acetonitrile/ethanol/DCM then it is very stable and shows full 
response on HPLC but i cannot grow my organism in such organic solvents it 
will die. It has to be a proper mixture of Broth (BG11) + pesticide in ppm. If 
you dissolve pesticide in organic solvent and then add it to broth, the broth 
turns milky and instantly chlorpyrifos forms TCP So, bioremediation experiment 
cannot be studied.

Reason:
I am pursuing research. I am referring to various papers.But, i dont get a 
proper methodology as to how to get Chlorpyrifos dissolved in liquid media to 
check for degradation by cyanobacteria. Predominantly, papers for soil media 
are available.

Thankyou very much.

Re: How to make 10-120ppm of Chlorpyrifos (Technical grade insecticide) to chec