MadSci Network: Biochemistry |
I did an experiemnt which explore different methods for precipitating protein from aqueous solution but I haven't found any info on how they make the protein precipiate. agents tht i used: 5 ml of 95% ethanol. 5 ml of 25% ammonium sulphate solution solid ammonium sulphate 5 ml of 5% trichloracetic acid 5 ml of 10% perchloric acid 5 ml of 2.5% perchloric acid 5 ml 10% w/v sodium tungstate in 0.2M H2SO4. 5 ml of Uranyl acetate solution. 0.3 ml of 0.15M barium hydroxide with 0.3 ml of 5% zinc sulphate. protein used in this experiment: serum albumin i hope you would be able to help me and if there is any books/websites tht would provide this info plz let me know.
Re: How does these precipitating agents works?
Try the links in the MadSci Library for more information on Biochemistry.