MadSci Network: Chemistry

Re: Why dilute the analyte?

Date: Fri May 8 01:17:36 2009
Posted By: Sebastien De Landtsheer, Undergraduate, Immunology, Laboratoire National de Santé
Area of science: Chemistry
ID: 1238357441.Ch


All analysis methods, even titrations, are precise and correct only within a certain range of analyte concentration. If your analyte is very concentrated, you will never titrate it (imagine neutralysing HCl 35% with NaOH 0.01M; it takes time). If it is not concentrated enough (as in the case of NaOH 0.01M with HCl), a drop will be sufficient and you end up having data lost in the background. Your analyte should be diluted in order to fit into the range of concentrations where your method is optimal.

Without more information about your manipulation I cannot answer your question further.

Hope that helps...

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