Subject: Why will transformed bacteria only contain one type of construct?
Date: Wed Jun 3 06:52:25 2009
Posted by Tina
Grade level: teacher/prof
School: University of Innsbruck
City: Innsbruck State/Province: No state entered.
Area of science: Molecular Biology
On the mystery of transformation of E. coli: If I expose competent cells to a
mixture of different constructs (i.e., a ligation reaction that will contain
religands and vector with insert), how do I always get 'monoclonal' colonies (I
never observe preps containing religand AND vector with insert, although each of
them can show up separately)? Thanks.
Re: Why will transformed bacteria only contain one type of construct?
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