Subject: Why will transformed bacteria only contain one type of construct?
Date: Wed Jun 3 06:52:25 2009
Posted by Tina
Grade level: teacher/prof
School: University of Innsbruck
City: Innsbruck State/Province: No state entered.
Country: Austria
Area of science: Molecular Biology
ID: 1244037145.Mb
Message:
On the mystery of transformation of E. coli: If I expose competent cells to a
mixture of different constructs (i.e., a ligation reaction that will contain
religands and vector with insert), how do I always get 'monoclonal' colonies (I
never observe preps containing religand AND vector with insert, although each of
them can show up separately)? Thanks.
Re: Why will transformed bacteria only contain one type of construct?
Current Queue |
Current Queue for Molecular Biology |
Molecular Biology archives
Try the links in the MadSci Library for more information on Molecular Biology.
MadSci Home | Information |
Search |
Random Knowledge Generator |
MadSci Archives |
Mad Library | MAD Labs |
MAD FAQs |
Ask a ? |
Join Us! |
Help Support MadSci
MadSci Network,
webadmin@madsci.org
© 1995-2006. All rights reserved.