How can a contour length of an isolated covalently closed circular (ccc) DNA molecule be measured?

Subject: How can a contour length of an isolated covalently closed circular (ccc) DNA molecule be measured?

Date: Mon Jun 8 06:59:37 2009
Posted by Nina
Grade level: undergrad School: Faculty of Science, Charles University
City: Prague State/Province: No state entered. Country: Czech Republic
Area of science: Molecular Biology
ID: 1244469577.Mb

Message:


Are there any standards for comparison? Because it is a covalently closed circular (ccc) molecule, 
I can't measure the contour length as it is in the microscope. I've read more, and I think there is 
some connection to open circular (oc) DNA form. Does that mean I should nick the ccc DNA 
molecule to oc DNA molecule and then measure the length? Thank you

Re: How can a contour length of an isolated covalently closed circular (ccc) DNA molecule be measured?

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