Subject: when I extract proline form plant tissue I dont know why most of the red c

when I prepared plant extracts in 3% sulphosalicylic acid  I saw the red color 
is thiat is o.k, but what happend when I add a mixture of acid-ninhydrin and 
acetic acid to 90 c, for 1h  and extracted the reaction mixture with 
toluene not all the color present in the toluene phase, I dont know why most 
of the red colore still in water phase and not in toluene phase

Re: when I extract proline form plant tissue I dont know why most of the red c