MadSci Network: Molecular Biology
Query:

Re: What is the major difference between subcloning and molecular cloning?

Date: Mon May 17 19:39:05 2010
Posted By: Sanjida Rangwala, Ohio State University
Area of science: Molecular Biology
ID: 1273844492.Mb
Message:

Sub-cloning is when you take something that you've already cloned (which is what
you've been doing) and then clone it into something else. For instance, you
might have a gene in a BAC, but then you cut it out using restriction enzymes
and ligate it into pBluescript vector. 

Hope this helps!

Sanjida


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