|MadSci Network: Molecular Biology|
As far as my understanding, chromosome walking is performed by taking a clone of known sequence, that we know lies near some region of interest, to probe genomic library generates by different restriction enzyme. In this way, we will get two clones that will hybridize with the probe. Both of them have overlapping sequence with the first probe. Then, one of them is used to probe the genomic library where the first probe is derived from. Similarly, two clones shows hybridization (one is the 1st clone used and the other will be new overlapping clone). Then, the newly overlapping clone is used as probe for the 2nd library again. This is repeated gradually building up a partial restriction map, until we reach the particular locus of interest. But, what's all this about? To sequence the locus of interest? or just to identify the surrounding sequence of a particular known gene? Is there any obvious pratical example for this? thank you.
Re: What is the purpose of chromosome walking?
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