| MadSci Network: Botany |
Kelly, Bleach (sodium hypochlorite) is toxic. We tend to take it for granted because it is a household chemical. However, bleach is a strong oxidizer that can cause severe damage to living material. In fact, the toxicity of bleach is such that it is often used as a disinfectant, and microorganisms have not developed resistance. Algae and plants are no exception to the toxicity of bleach. This is why millions of people add sodium hypochlorite to their swimming pools and municipalities chlorinate the water supply. It is not the presence of chloride ions that makes bleach toxic, but the added oxygen in bleach. This is highly reactive and oxidizes most organic compounds. Oxidation of colored chemical compounds results in bleaching. Please seehttp://howstuffworks.com/ question189.htm for more detailed information. Remember plants and algae rely on a variety of colored compounds for their livelihood. These include chlorophyll, of course, but also carotenoids and other "antenna" pigments and protective pigments. When these are damaged or bleached-out, plants cannot photosynthesize. Sodium hypochlorite also produces hydrochloric acid when in solution. This acid has toxic effects all its own. For example chlorophyll is very sensitive to exposure to acid conditions. Dunaliella has been the subject of many, many studies in phycology and plant physiology. This is largely because it is the algal version of a lab rat. It is easy to grow, and seems to thrive under laboratory conditions which make it the ideal candidate for physiological research. I will not go into details of culture here. These are treated well in many publications. For easy access to information on algal culture I will refer you to the Phycological Society pages and links located there. http://www.psaalgae.org/l inks/links.html Another very valuable resource is the Provasoli-Guillard Center for the Culture of Marine Phytoplankton. Their site has a wealth of information on algal culture including culture conditions and media recipes and is available at: http://ccmp.bigelow.org/ Hopefully helpful.
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