| MadSci Network: Chemistry |
Analysis of Ascorbic Acid or Vitamin C usually involves oxidation by a redox dye which in your method's case was iodine. This method measures the amount of Vitamin C that is avaliable to be reduced. This method only measures some forms of Vitamin C and not all of them so you will only get a measure of some of the Vitamin C in the food. There are more complex methods used to day for research and by the food companies to tell the consumer exactly how much vitamin C is found in fruit juices. I work with an HPLC (High Performance Liquid Chromatography method). This method measures all the forms of Vitamin C that are used by the body for this vitamin. Usually, people don't realize that when we talk about vitamins we are talking about several chemicals that are used by the body for Vitamin C. (The different forms are: Ascorbic Acid, Isoascorbic Acid, Dehydro-L-Ascorbic Acid, and Dehydro-D-Isoascorbic Acid) I am sure that your method only measured the AA form. The procedure that I am more familiar with is a reaction that appears to be the reverse of what happened in your reaction. A dye (2,6-Dichloropenol-indopenol) which is blue is actuallyreduced by Ascorbic Acid (AA) to a colorless form with more Ascorbic Acid.
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