MadSci Network: Biochemistry
Query:

Subject: Why does adenine have more mobility in a chromatograph then cytosine?

Date: Sun Oct 18 22:13:39 1998
Posted by Jeremy Herring
Grade level: undergrad
School: University of Waterloo
City: Waterloo State/Province: Ontario
Country: Canada
Area of science: Biochemistry
ID: 908766819.Bc
Message:

In a biology lab, we were using chromatography to separate nucleic 
acids.  We found that adenine went farther along the sheet (had a 
higher Rf)then the cytosine.  Since adenine is a larger molecule, 
shouldn't it have moved less?  Is there a reason for this or is it 
simple experimental error?


Re: Why does adenine have more mobility in a chromatograph then cytosine?

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