Basic Techniques
- Lighting a bunsen burner.
- Sterilizing an inoculating loop
- How to streak agar plates.
- Inoculating agar slants.
- How to create a bacterial "lawn" on an agar plate.
- Disposal and proper autoclaving microbiological waste.
- Always wash your hands when done!
Intermediate
- Performing and interpreting serial dilutions.
- Preparing and pouring agar plates.
Advanced
- Performing and interpreting a Gram stain.
- Use of the microscope.
- Low-tech method for preparing anaerobic broth media.
Equipment
- Where to find/purchase equipment and reagents
- Protective gear: gloves, lab coat, safety goggles.
- Bunsen Burner
- Striker for lighting the burner.
- Non-flame heat source for sterilizing inoculating loops.
- Nichrome wire inoculating loops, disposable loops, and low-tech alternatives.
- Incubators.
- Autoclaves, and low(er)-tech alternatives.
- Pipettes and pipettors.