Solid media commonly contain 1.5% agar per weight to solidify the liquid, nutrient part of the media. After sterilization, the liquid media is poured into sterile Petrie plates - think of it along the lines of pouring heated jellow into small cylindrical molds.. Petrie plates are commonly made of plastic, and can be purchased already sterlilzed with 25 or more per package.
|Luria Bertani Broth (LB)
||Sheep's Blood Agar
||Hektoen Enteric Agar
|Uses: Culture of aerobic
||Uses: Culture of aerobic
bacterial species on solid media
||Uses: Selective media for
Gram-negative, enteric bacteria (bile tolerant). Assess lactose
||Uses: Non-selective media, good
for culturing many bacterial species including Gram negative and
Gram-positive species including Staphylococci and Streptococci
||Uses: Very enriched media with
lysed red cells, to release many nutrients needed by *fastidious*
It may look like chocolate, but would have a rather nasty flavor, like coagulated blood-flavored jello..
|Uses: Selective media for
Gram-negative enteric bacteria (higher bile salt concentrations than
Used to assess lactose fermentation AND ability to produce hydrogen sulfide (H2S). Allows for distinguishing among, E. coli, Salmonella and other gut pathogens including Shigella species
Distribute 5-10mL into glass tubes with autoclavable tops.
Autoclave 30 minutes, OR place in a pressure cooker for ~45 minutes @ 15 pounds pressure.
|Same as for LB-broth, but add
15g of agar prior to autoclaving.
Be certain to mix thoroughly prior to pouring media into Petrie plates.
||Recipe: Recommend commerically
||Recipe: Recommend commercially
If bought in powder form, must heat over heating plate prior to pouring plates. Do not autoclave.