| MadSci Network: Microbiology |
established direct email communication with requestor...following is copy of direct response: hi cortnee, my name is heather and i am responding to your mad scientist request....first of all don't touch the money with your bare fingers, as there are RNases in your skin so wear gloves and pick up the money with sterile forceps...do you have access to an autoclave to sterilize instruments? next you can take a sterile cotton swab...you can sterilize some Q-tips...swab the money and then rub it on the agar....what kind af agar are you using? you should make 3 plates of each if you can...one to store in a 37 C degree incubator, one at room temp (20-25 C) and one in the refrigerator (4 C) as some bacteria and fungi grow at diff temps...if you can make 2 plates of each temp just for backup would be good....make double sure the petri plates and agar are and remain sterile until you innoculate them...you can observe the bacterial and fungal growth this way...however, you will not be able to test for viruses...this is a very complicated procedure which most schools are not equiped to test...they require a living organism to grow in, they cannot be grown on agar plates....if you can get access to diff agar media that may be great also....if you have any more questions or would like to discuss anything just drop me a line...thanks....cya...H. Peace :)
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