MadSci Network: Molecular Biology |
I have noticed that some genes, such as those for collagen types 1 and 3, produce two mRNA bands of different sizes on northern blots. This is well documented in research publications, but no paper has discussed the significance of the two distinct mRNA products derived from a single gene. The double mRNA is detected regardless if the nucleic acids were derived from total RNA or purified mRNA thereby eliminating the possibility of detecting homologous nuclear RNA (hnRNA) My question is: are both of the RNA species detected true messenger RNA? Or is one of them (presumably the one with higher molecular weight)an unprocessed, or immature, mRNA that undergoes further processing into a more translatable mRNA? Thanks for your help, Richard Isbrucker
Re:2 different mRNA sizes for one gene
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