MadSci Network: Chemistry

Re: How can I easily determine the percentage of sucrose in a spice blend?

Date: Thu Nov 18 08:14:02 1999
Posted By: Alfred Bushway, Professor of Food Science
Area of science: Chemistry
ID: 941746532.Ch

Mr. Allen,

You have asked a question that does't have a simple answer based on the 
equipment you have avialable for analysis. The easiest method to determine 
sucrose content would be to extract the spice blend with 65-80% ethanol and 
than determine the sucrose content by High Performance Liquid 
Chromatography with a Refractive Index Detector.

One method you might try would be to extract your spice blend with 
deionized water, filter and measure the percent soluble solids with your 
refractometer. You would need to correct for the percent salt in the blend 
since this will contribute to the soluble solids content. Assuming the 
amount of sodium glutamate and other water extractives are small while the 
sucrose content is significantly higher this would give you an 
approixmation of the sucrose content in the blend.

There are colrimetric methods for measuring sucrose, but they would require 
hydrolsis to monosachrides and/or enzyme linked assays.One company does 
manufacutre kits for the UV determination of mono- & disaccarides in 
foodstuffs. The company is Boehringer Mannheim Biochemicals which is 
located in Indianapolis, IN (address - 7941 Castleway Drive, P.O. Box 
50816, Indianapolis, IN  46250). These are not simple and can require 
consiberable time.

I hope you find this response of some assistance.

Al Bushway
Professor of Food Science

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