MadSci Network: General Biology |
Your effort is interesting. I have some points to tell you that will affect your work. First of all the sources of water input for the body are 2: oral intake and water produced in metabolism. However the sources of output are many such as loss thru skin, loss of water during respiration, sweat, feces, and lastly urine output. During your experimental work you will have to keep this in mind that whatever you take in orally will not appear equally in urine and the problem you will face is that you won't be able to calculate the water loss through other sources. When completely at rest, the urine output is expected to increase propotionally with increase in water intake. In the other case if you maintain a constant exercise (activity) level instead of rest, the urine output will still increase in proportion. However it may practically be very difficult for you to maintain the constant activity level while you take different quantities of water and failing to do so will alter the results as inconsistency in exercise will cause changes in loss of water through sweat. If more water is lost in sweat in one case than other then the change in urine output will not be as expected. That is the sweat water loss should be constant throughout the experimentation when you want to study the water loss accompanied by exercise. Another option could be that first you can calculate the changes in urine output in relation to changes in water intake at complete rest. Then with changing intake on the same pattern right from the beginning but altering exercise level, you could show that the water loss in urine does not increase as expected due to exercise as the water loss is diverted to sweat. Now we come to the expected results; when you study distilled water you will observe increases in urine output with increases in water intake. However if you study hypertonic solution (compared to plasma) the urine output is supposed to decrease. Its important to note that the solution should be hypertonic than plasma i.e. body's fluid medium. Any other solution that you are considering hypertonic that is actually hypotonic compared to plasma will not give you decreesed urine output as it is still adding water to body being hypotonic than plasma.
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