MadSci Network: Biochemistry
Query:

Re: How can I change the pH of the decomposition reaction of hydrogen peroxide?

Date: Mon Sep 25 12:19:47 2000
Posted By: Peter Traill, Undergraduate, Chemistry, University of Dundee
Area of science: Biochemistry
ID: 969269599.Bc
Message:

Dear Stuart,
 I know this project well, I did it myself in the final year of High School last 
century. To change the pH you might need.
 acid (0.1M hydrochloric acid)
 alkali (0.1M sodium hydroxide)
 The buffers
 test tubes (quite a few large ones)
 measuring cylinders (two, one for acid one for alkali)
 electronic pH meter
 
Ok, firstly you set up the solutions:
 in test tube one put in about 10 ml of acid, and 2 ml of pH 2 buffer (check 
using the pH meter, if different add acid/alkali to get to the level)
 then in the rest
 2: 9ml acid, 1ml alkali, 2ml pH3 buffer
 3: 8ml acid . 2ml alkali, 2ml pH4 buffer
 4: 7ml acid, 3ml alakali, 2ml pH5 buffer, 
and so on......

 that should give you nine test tubes with different pHs in them for you to 
conduct the experiments.

Good luck with the project and thanks for asking. And have you tried 
changing the concentration of enzyme ?

yours scientifically,
Peter D. Traill


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