|MadSci Network: Molecular Biology|
I am interested in making a transgenic plant for a school project. The method that I came up involves removing the cell wall and then exposing it to CaCl2 to open up the cell membrane making it accept foriegn DNA. I have been told that this is the method used in the 80's and 90's. It seems it would be easier to use Agrobacterium to insert forieng DNA because the plant cell wall would not have to be removed. Unfortunately, I have not found a way to get Agrobacterium. Does this mean it is not available unless one has special access? Also, the National Institute of Health has its biosafety regulations. This seems like it would fall under these regulations. However, I have seen catalogs with kits for creating transgenic bacteria and some even using altered strains of E. coli. It seems confusing that these kits could be sold without confirmation of biosafety qualifications met. Thank you for your time.
Re: Creating a transgenic plant
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