MadSci Network: General Biology |
I realize a glitch in the answering system may have delayed this beyond usefulness, but here is your answer... You can measure phytoplankton concentration using a spectrometer that measures absorbance of visible light. Find out which wavelength would be suitable for green or red, and measure there. Take a background reading of the water or growth medium alone and zero the instrument, then take a measurement of your phytoplankton culture. The spectrometer we commonly use for this is called a Spec20, and most high schools have this or a similar piece of equipment. You will need to transfer some of your culture to a test tube to perform the measurement (unless you have a flask with a test tube arm). You can sterilize the test tube with alcohol beforehand to prevent contamination (70% ethanol in water - swirl around for 30 seconds, then dump and allow to dry). This way, you can minimize contamination when you pour the culture back into the larger flask. Good luck!
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