MadSci Network: Zoology

Re: What concentration and dosage of epinephrine can a goldfish be exposed to?

Date: Mon Apr 22 10:02:24 2002
Posted By: David Mallory, Faculty, Biological Sciences, Marshall University
Area of science: Zoology
ID: 1019424090.Zo


I have seen this experiment also performed on the webbing between a frog's 
toes, on the blood vessels in the frog urinary bladder and on the blood 
vessels in the mesentary (thin support tissues) around the intestines. In 
each study the concentration of epinephrine was between 1 microgram/mL (1 
mcg/ml) and 10 mcg/ml. (J. Woodring and T.H. Dietz: Laborartory Manual 
for Human Physiology, 2nd ed. 1992. Saunders College Publishing.  pp 
116.; G.D. Tharp: Experiments in Physiology, 7th ed. Prentice Hall 
Publishers.1997. pp 219. Most common supplies of epinephrine are made to a 
concentration of 1 miligram/ml (1mg/ml), so you will have to do a 1:100 
dilution to achieve 10mcg/ml or a 1:1000 dilution to achieve a 1mcg/ml 
working solution. 

You should be aware that the goldfish will be stressed merely by taking 
them from the water, however, the stress can be minimized by being quiet, 
handling them gently and making your observations quickly.

Good Luck!

David Mallory, Ph.D.
Associate Professor
Department of Biological Sciences
Marshall University
Huntington, WV 25755-2510

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