|MadSci Network: Zoology|
Krista: I have seen this experiment also performed on the webbing between a frog's toes, on the blood vessels in the frog urinary bladder and on the blood vessels in the mesentary (thin support tissues) around the intestines. In each study the concentration of epinephrine was between 1 microgram/mL (1 mcg/ml) and 10 mcg/ml. (J. Woodring and T.H. Dietz: Laborartory Manual for Human Physiology, 2nd ed. 1992. Saunders College Publishing. pp 116.; G.D. Tharp: Experiments in Physiology, 7th ed. Prentice Hall Publishers.1997. pp 219. Most common supplies of epinephrine are made to a concentration of 1 miligram/ml (1mg/ml), so you will have to do a 1:100 dilution to achieve 10mcg/ml or a 1:1000 dilution to achieve a 1mcg/ml working solution. You should be aware that the goldfish will be stressed merely by taking them from the water, however, the stress can be minimized by being quiet, handling them gently and making your observations quickly. Good Luck! David Mallory, Ph.D. Associate Professor Department of Biological Sciences Marshall University Huntington, WV 25755-2510
Try the links in the MadSci Library for more information on Zoology.