MadSci Network: Molecular Biology

Subject: Can the plasmid influence itself the function of a gene?

Date: Thu Jul 17 08:16:52 2003
Posted by helder
Grade level: grad (science) School: university of Leiden
City: Leiden State/Province: No state entered. Country: Netherlands
Area of science: Molecular Biology
ID: 1058447812.Mb

 Dear Mr.(s) I am student doing a work in yeast genetics. I clone a gene( 
FPR3) into a plasmid pRS313 and did a trasnformation in a strain that has that 
gene FPR3 deleted. The strange thing is because i am studing the genes 
involved in the subcellular localization of WSs1 protein. I verified that when 
the gene FPR3 is deleted from the genome the WSs1 localization is altered. So 
my intent is to clone taht gene into the pRS313 and transform the yeast cell. 
I did a restriction of cloned plasmid inspecific enzymes showed that the 
fragment is not integrated on the plasmid but i did the trasformation anyway 
and the strange was that the wild type localization of Wss1p returns. How can 
it be possible? I did not a control using only the trasnformation with pRS313 
but can be the result due only to the plasmid? 
Thank you very much for your help. 
Best regards 
Helder Simao

Re: Can the plasmid influence itself the function of a gene?

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