|MadSci Network: Molecular Biology|
Dear Mr.(s) I am student doing a work in yeast genetics. I clone a gene( FPR3) into a plasmid pRS313 and did a trasnformation in a strain that has that gene FPR3 deleted. The strange thing is because i am studing the genes involved in the subcellular localization of WSs1 protein. I verified that when the gene FPR3 is deleted from the genome the WSs1 localization is altered. So my intent is to clone taht gene into the pRS313 and transform the yeast cell. I did a restriction of cloned plasmid inspecific enzymes showed that the fragment is not integrated on the plasmid but i did the trasformation anyway and the strange was that the wild type localization of Wss1p returns. How can it be possible? I did not a control using only the trasnformation with pRS313 but can be the result due only to the plasmid? Thank you very much for your help. Best regards Helder Simao
Re: Can the plasmid influence itself the function of a gene?
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