MadSci Network: Molecular Biology |
I would say it is unlikely that the plasmid alone could affect the cells in the way you described, but without doing the control transformation (with the pRS313 plasmid alone) it is impossible to conclude anything. Are you sure that the cells you used were FPR3 deleted? The only option you have is to repeat the experiment again with the correct controls and see what happens. If the same result occurs then you can look into it in more detail. Good luck!
Try the links in the MadSci Library for more information on Molecular Biology.